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hfsc serum free un differentiation media  (Celprogen Inc)


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    Structured Review

    Celprogen Inc hfsc serum free un differentiation media
    Figure 5. Hair follicle stem cell <t>(HFSC)</t> TLR2 is crucial for wound-induced hair follicle regeneration. (A) Schematic of wound healing assay using TLR2- GFP reporter mouse. Full-thickness wounds on the dorsal skin of TLR2-GFP mice were created. Normal unwounded skin and the skin adjacent to the wound were harvested at different time points. (B) Representative confocal images of normal and wounded skin from TLR2-GFP mice at different time points post-injury immunostained for TLR2-GFP and CD34. Scale bars are 20 μm. (C) Quantification of TLR2 fluorescent intensity per bulge cell in hair
    Hfsc Serum Free Un Differentiation Media, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hfsc+serum+free+un+differentiation+media/Human+Hair+Follicle+Stem+Cell+Serum+Free+Un-differentiation+Media/10__7554_slash_elife__89335-379-13-19
    Average 93 stars, based on 1 article reviews
    hfsc serum free un differentiation media - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "TLR2 regulates hair follicle cycle and regeneration via BMP signaling"

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling

    Journal: eLife

    doi: 10.7554/elife.89335

    Figure 5. Hair follicle stem cell (HFSC) TLR2 is crucial for wound-induced hair follicle regeneration. (A) Schematic of wound healing assay using TLR2- GFP reporter mouse. Full-thickness wounds on the dorsal skin of TLR2-GFP mice were created. Normal unwounded skin and the skin adjacent to the wound were harvested at different time points. (B) Representative confocal images of normal and wounded skin from TLR2-GFP mice at different time points post-injury immunostained for TLR2-GFP and CD34. Scale bars are 20 μm. (C) Quantification of TLR2 fluorescent intensity per bulge cell in hair
    Figure Legend Snippet: Figure 5. Hair follicle stem cell (HFSC) TLR2 is crucial for wound-induced hair follicle regeneration. (A) Schematic of wound healing assay using TLR2- GFP reporter mouse. Full-thickness wounds on the dorsal skin of TLR2-GFP mice were created. Normal unwounded skin and the skin adjacent to the wound were harvested at different time points. (B) Representative confocal images of normal and wounded skin from TLR2-GFP mice at different time points post-injury immunostained for TLR2-GFP and CD34. Scale bars are 20 μm. (C) Quantification of TLR2 fluorescent intensity per bulge cell in hair

    Techniques Used: Wound Healing Assay

    Figure 6. Oxidation-dependent TLR2 ligand carboxyethylpyrrole (CEP) is present in hair follicles and promotes hair regeneration via hair follicle stem cell (HFSC) TLR2. (A) Representative images of H&E and CEP immunostaining of consecutive skin sections from wild-type (WT) anagen mouse. Scale bars are 1 mm. (B) Representative confocal images of P5 WT whole-mount skin immunostained for CEP and Ker17. The merged image shows the co- localization of CEP to anagen hair follicles (Ker17+). Scale bar is 200 μm. (C) Longitudinal and cross-sections of anagen and telogen hair follicles from
    Figure Legend Snippet: Figure 6. Oxidation-dependent TLR2 ligand carboxyethylpyrrole (CEP) is present in hair follicles and promotes hair regeneration via hair follicle stem cell (HFSC) TLR2. (A) Representative images of H&E and CEP immunostaining of consecutive skin sections from wild-type (WT) anagen mouse. Scale bars are 1 mm. (B) Representative confocal images of P5 WT whole-mount skin immunostained for CEP and Ker17. The merged image shows the co- localization of CEP to anagen hair follicles (Ker17+). Scale bar is 200 μm. (C) Longitudinal and cross-sections of anagen and telogen hair follicles from

    Techniques Used: Immunostaining

    Related Articles

    Incubation:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Wound Healing Assay:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Immunostaining:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Staining:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    MANN-WHITNEY:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Irradiation:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Transplantation Assay:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Isolation:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Cell Culture:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Real-time Polymerase Chain Reaction:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Purification:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Activity Assay:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Knock-In:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Sequencing:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Blocking Assay:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Concentration Assay:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Membrane:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Recombinant:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.

    Software:

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: Human HFSCs (Celprogen cat.# 36007-08) were cultured in HFSC Un-differentiation Media with Serum (Celprogen cat.# M36007-08US) for 48 hr and then transferred into Undifferentiated ECM 96-Well Plates (Celprogen cat.# UD36007-08-96Well) for 24 hr.

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr.



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    Celprogen Inc hfsc serum free un differentiation media
    Figure 5. Hair follicle stem cell <t>(HFSC)</t> TLR2 is crucial for wound-induced hair follicle regeneration. (A) Schematic of wound healing assay using TLR2- GFP reporter mouse. Full-thickness wounds on the dorsal skin of TLR2-GFP mice were created. Normal unwounded skin and the skin adjacent to the wound were harvested at different time points. (B) Representative confocal images of normal and wounded skin from TLR2-GFP mice at different time points post-injury immunostained for TLR2-GFP and CD34. Scale bars are 20 μm. (C) Quantification of TLR2 fluorescent intensity per bulge cell in hair
    Hfsc Serum Free Un Differentiation Media, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hfsc+serum+free+un+differentiation+media/Human+Hair+Follicle+Stem+Cell+Serum+Free+Un-differentiation+Media/10__7554_slash_elife__89335-379-13-19
    Average 93 stars, based on 1 article reviews
    hfsc serum free un differentiation media - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

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    Figure 5. Hair follicle stem cell (HFSC) TLR2 is crucial for wound-induced hair follicle regeneration. (A) Schematic of wound healing assay using TLR2- GFP reporter mouse. Full-thickness wounds on the dorsal skin of TLR2-GFP mice were created. Normal unwounded skin and the skin adjacent to the wound were harvested at different time points. (B) Representative confocal images of normal and wounded skin from TLR2-GFP mice at different time points post-injury immunostained for TLR2-GFP and CD34. Scale bars are 20 μm. (C) Quantification of TLR2 fluorescent intensity per bulge cell in hair

    Journal: eLife

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling

    doi: 10.7554/elife.89335

    Figure Lengend Snippet: Figure 5. Hair follicle stem cell (HFSC) TLR2 is crucial for wound-induced hair follicle regeneration. (A) Schematic of wound healing assay using TLR2- GFP reporter mouse. Full-thickness wounds on the dorsal skin of TLR2-GFP mice were created. Normal unwounded skin and the skin adjacent to the wound were harvested at different time points. (B) Representative confocal images of normal and wounded skin from TLR2-GFP mice at different time points post-injury immunostained for TLR2-GFP and CD34. Scale bars are 20 μm. (C) Quantification of TLR2 fluorescent intensity per bulge cell in hair

    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFSC Serum Free Un- differentiation Media (Celprogen cat.# M36007- 08U) overnight.

    Techniques: Wound Healing Assay

    Figure 6. Oxidation-dependent TLR2 ligand carboxyethylpyrrole (CEP) is present in hair follicles and promotes hair regeneration via hair follicle stem cell (HFSC) TLR2. (A) Representative images of H&E and CEP immunostaining of consecutive skin sections from wild-type (WT) anagen mouse. Scale bars are 1 mm. (B) Representative confocal images of P5 WT whole-mount skin immunostained for CEP and Ker17. The merged image shows the co- localization of CEP to anagen hair follicles (Ker17+). Scale bar is 200 μm. (C) Longitudinal and cross-sections of anagen and telogen hair follicles from

    Journal: eLife

    Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling

    doi: 10.7554/elife.89335

    Figure Lengend Snippet: Figure 6. Oxidation-dependent TLR2 ligand carboxyethylpyrrole (CEP) is present in hair follicles and promotes hair regeneration via hair follicle stem cell (HFSC) TLR2. (A) Representative images of H&E and CEP immunostaining of consecutive skin sections from wild-type (WT) anagen mouse. Scale bars are 1 mm. (B) Representative confocal images of P5 WT whole-mount skin immunostained for CEP and Ker17. The merged image shows the co- localization of CEP to anagen hair follicles (Ker17+). Scale bar is 200 μm. (C) Longitudinal and cross-sections of anagen and telogen hair follicles from

    Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFSC Serum Free Un- differentiation Media (Celprogen cat.# M36007- 08U) overnight.

    Techniques: Immunostaining