hfsc serum free un differentiation media (Celprogen Inc)
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Hfsc Serum Free Un Differentiation Media, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hfsc+serum+free+un+differentiation+media/Human+Hair+Follicle+Stem+Cell+Serum+Free+Un-differentiation+Media/10__7554_slash_elife__89335-379-13-19
Average 93 stars, based on 1 article reviews
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1) Product Images from "TLR2 regulates hair follicle cycle and regeneration via BMP signaling"
Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling
Journal: eLife
doi: 10.7554/elife.89335
Figure Legend Snippet: Figure 5. Hair follicle stem cell (HFSC) TLR2 is crucial for wound-induced hair follicle regeneration. (A) Schematic of wound healing assay using TLR2- GFP reporter mouse. Full-thickness wounds on the dorsal skin of TLR2-GFP mice were created. Normal unwounded skin and the skin adjacent to the wound were harvested at different time points. (B) Representative confocal images of normal and wounded skin from TLR2-GFP mice at different time points post-injury immunostained for TLR2-GFP and CD34. Scale bars are 20 μm. (C) Quantification of TLR2 fluorescent intensity per bulge cell in hair
Techniques Used: Wound Healing Assay
Figure Legend Snippet: Figure 6. Oxidation-dependent TLR2 ligand carboxyethylpyrrole (CEP) is present in hair follicles and promotes hair regeneration via hair follicle stem cell (HFSC) TLR2. (A) Representative images of H&E and CEP immunostaining of consecutive skin sections from wild-type (WT) anagen mouse. Scale bars are 1 mm. (B) Representative confocal images of P5 WT whole-mount skin immunostained for CEP and Ker17. The merged image shows the co- localization of CEP to anagen hair follicles (Ker17+). Scale bar is 200 μm. (C) Longitudinal and cross-sections of anagen and telogen hair follicles from
Techniques Used: Immunostaining
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Incubation:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Wound Healing Assay:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Immunostaining:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Staining:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. MANN-WHITNEY:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Irradiation:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Transplantation Assay:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Isolation:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Cell Culture:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Real-time Polymerase Chain Reaction:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Purification:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Activity Assay:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Knock-In:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Sequencing:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Blocking Assay:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Concentration Assay:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Membrane:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Recombinant:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. Software:Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: Article Title: TLR2 regulates hair follicle cycle and regeneration via BMP signaling Article Snippet: After 24 hr cells were washed with 1× D- PBS and incubated in HFDPC Basal Medium contains no growth supplement (Cell Applications, Inc cat.# 610- 500) for the next 24 hr. |